fluorescein isothiocyanate conjugated avidin fitc avidin  (Vector Laboratories)

Journal: Frontiers in Neuroanatomy

Article Title: Morphological investigations of endomorphin-2 and spinoparabrachial projection neurons in the spinal dorsal horn of the rat

doi: 10.3389/fnana.2022.1072704

Figure Lengend Snippet: Antisera used in each group for light microscopy.

Article Snippet: These sections were blocked with 10% normal donkey serum (NDS) for 0.5 h, then subjected to the following incubations with: (1) primary antisera in the dilution medium [5% NDS, 0.3% Triton X-100, 0.05% NaN3 and 0.25% λ-carrageenan in 0.01 M PBS (PBS-NDS, pH 7.4)] overnight at room temperature (RT) and then 72 h at 4°C; (2) secondary antisera in PBS-NDS for 6 h at RT; (3) FITC-conjugated avidin (A-2001, Vector; Burlingame, CA, USA) in PBS containing 0.3% Triton X-100 (PBS-X, pH 7.4) for 3 h at RT.

Techniques: Microscopy